Coding

Part:BBa_K137017:Design

Designed by: Doug Tischer   Group: iGEM08_Caltech   (2008-07-15)


Galactose Oxidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1294
    Illegal NotI site found at 517
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1424
    Illegal BamHI site found at 144
    Illegal BamHI site found at 589
    Illegal BamHI site found at 624
    Illegal BamHI site found at 782
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Two rounds of site directed mutagenesis had to be performed to eliminate an EcoRI and PstI site.


Source

Plasmid from the Arnold lab at CalTech, which is the result of their directed evolution to make galactose functionally expressed in E. coli and to improve its thermostability.

References